This set of Petri dishes already has had all of that done for you so all you need to do is put the micro organisms in and wait for them to grow. It's very convenient but there is extra cost because of this. I was quite pleased with the dishes I received; they were well-packaged, free of contamination, and most definitely ready to go. The package advises you to put the dishes in a 4 C environment when you get them this turns out to be the temperature of my fridge... don't put them in the freezer as I did with my first set! The water in the agar freezes and when you pull the dishes out to use them, you have to wait for them to defrost and when they do, you're left with a huge number of cracks in the agar, rendering most of the plate unusable...
If you take care not to repeat my mistake, these should work well for you. If you need large numbers of Petri dishes, consider buying powder to mix your own nutrient agar to reduce costs.
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I have a PH.D. in biochemistry and have made and used my own agar plates for years in a research lab. Now I am a "retired" stay-at-home" mom and use these plates to do science experiments with my kids at home. They are great! I haven't had any problems with them at all. They work just like they should. You don't really need to refrigerate them upon arrival if you are going to be using them within a week or so. I prefer not to refrigerate mine because IF they are contaminated, I want to KNOW IT and SEE the contamination before I use the plate. It is important to set up a warm "incubator" to keep them and they will grow over night. I bought a heat lamp (for reptiles sold at a pet store) and clipped it on the the side of a plastic box. I put a thermometer in there to make sure the temp stays between 90-98 degrees F. I covered it loosely with aluminum foil. That is all you really need to do. You should not let them grow more than 24 hours. Letting them grow for weeks as some people did will not generate accurate results. The plates will grow mold and dry out.
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